Load raw data, annotate probes using biomaRt and load SFARI genes
# Load csvs
datExpr = read.csv('./../raw_data/RNAseq_ASD_datExpr.csv', row.names=1)
datMeta = read.csv('./../raw_data/RNAseq_ASD_datMeta.csv')
SFARI_genes = read_csv('./../working_data/SFARI_genes_01-15-2019.csv')
# Make sure datExpr and datMeta columns/rows match
rownames(datMeta) = paste0('X', datMeta$Dissected_Sample_ID)
if(!all(colnames(datExpr) == rownames(datMeta))){
print('Columns in datExpr don\'t match the rowd in datMeta!')
}
# Annotate probes
getinfo = c('ensembl_gene_id','external_gene_id','chromosome_name','start_position',
'end_position','strand','band','gene_biotype','percentage_gc_content')
mart = useMart(biomart='ENSEMBL_MART_ENSEMBL',
dataset='hsapiens_gene_ensembl',
host='feb2014.archive.ensembl.org') ## Gencode v19
datProbes = getBM(attributes=getinfo, filters=c('ensembl_gene_id'), values=rownames(datExpr), mart=mart)
datProbes = datProbes[match(rownames(datExpr), datProbes$ensembl_gene_id),]
datProbes$length = datProbes$end_position-datProbes$start_position
# Group brain regions by lobes
datMeta$Brain_Region = as.factor(datMeta$Region)
datMeta$Brain_lobe = 'Occipital'
datMeta$Brain_lobe[datMeta$Brain_Region %in% c('BA4_6', 'BA9', 'BA24', 'BA44_45')] = 'Frontal'
datMeta$Brain_lobe[datMeta$Brain_Region %in% c('BA3_1_2_5', 'BA7')] = 'Parietal'
datMeta$Brain_lobe[datMeta$Brain_Region %in% c('BA38', 'BA39_40', 'BA20_37', 'BA41_42_22')] = 'Temporal'
datMeta$Brain_lobe=factor(datMeta$Brain_lobe, levels=c('Frontal', 'Temporal', 'Parietal', 'Occipital'))
#################################################################################
# FILTERS:
# 1 Filter probes with start or end position missing (filter 5)
# These can be filtered without probe info, they have weird IDS that don't start with ENS
to_keep = !is.na(datProbes$length)
datProbes = datProbes[to_keep,]
datExpr = datExpr[to_keep,]
rownames(datProbes) = datProbes$ensembl_gene_id
# 3. Filter samples from ID AN03345 (filter 2)
to_keep = (datMeta$Subject_ID != 'AN03345')
datMeta = datMeta[to_keep,]
datExpr = datExpr[,to_keep]
#################################################################################
# Annotate SFARI genes
# Get ensemble IDS for SFARI genes
mart = useMart(biomart='ENSEMBL_MART_ENSEMBL',
dataset='hsapiens_gene_ensembl',
host='feb2014.archive.ensembl.org') ## Gencode v19
gene_names = getBM(attributes=c('ensembl_gene_id', 'hgnc_symbol'), filters=c('hgnc_symbol'),
values=SFARI_genes$`gene-symbol`, mart=mart) %>%
mutate('gene-symbol'=hgnc_symbol, 'ID'=as.character(ensembl_gene_id)) %>%
dplyr::select('ID', 'gene-symbol')
SFARI_genes = left_join(SFARI_genes, gene_names, by='gene-symbol')
datExpr_backup = datExpr
print('SFARI genes count by score')
## [1] "SFARI genes count by score"
table(SFARI_genes$`gene-score`)
##
## 1 2 3 4 5 6
## 29 82 209 538 191 25
print('Samples count by lobe')
## [1] "Samples count by lobe"
table(datMeta$Brain_lobe)
##
## Frontal Temporal Parietal Occipital
## 21 20 22 23
rm(getinfo, to_keep, gene_names, mart)
Regions: Frontal, Temporal, Parietal and Occipital
y axis cut at 1000 to remove outliers
The distributions by score seem very similar between regions
make_ASD_vs_CTL_df = function(datExpr, lobe){
datMeta_lobe = datMeta %>% filter(Brain_lobe==lobe & rownames(datMeta) %in% colnames(datExpr))
datExpr_ASD = datExpr %>% data.frame %>% dplyr::select(which(datMeta_lobe$Diagnosis_=='ASD'))
datExpr_CTL = datExpr %>% data.frame %>% dplyr::select(which(datMeta_lobe$Diagnosis_!='ASD'))
ASD_vs_CTL = data.frame('ID'=as.character(rownames(datExpr)),
'mean_ASD'=rowMeans(datExpr_ASD), 'mean_CTL'=rowMeans(datExpr_CTL),
'sd_ASD'=apply(datExpr_ASD,1,sd), 'sd_CTL'=apply(datExpr_CTL,1,sd)) %>%
mutate('mean_diff'=mean_ASD-mean_CTL, 'sd_diff'=sd_ASD-sd_CTL) %>%
left_join(SFARI_genes, by='ID') %>%
dplyr::select(ID, mean_ASD, mean_CTL, mean_diff, sd_ASD, sd_CTL, sd_diff, `gene-score`) %>%
mutate('gene-score'=ifelse(is.na(`gene-score`),'None',`gene-score`))
return(ASD_vs_CTL)
}
p = list()
for(lobe in names(table(datMeta$Brain_lobe))){
datExpr_lobe = datExpr %>% dplyr::select(which(datMeta$Brain_lobe==lobe))
ASD_vs_CTL = make_ASD_vs_CTL_df(datExpr_lobe, lobe)
plot = ggplotly(ggplot(ASD_vs_CTL, aes(`gene-score`, abs(mean_diff), fill=`gene-score`)) +
geom_boxplot() + theme_minimal() + ylim(0, 1000) +
scale_fill_manual(values=gg_colour_hue(7)) +
theme(legend.position = 'none'))
p[lobe] = list(plot)
}
subplot(p[[1]], p[[2]], p[[3]], p[[4]], nrows=2)
rm(p, lobe, datExpr_lobe)
datExpr = datExpr_backup # Should be the same, but in case code is ran in different order...
# Conditional Quantile Normalisation (CQN)
cqn.dat = cqn(datExpr, lengths = datProbes$length,
x = datProbes$percentage_gc_content,
lengthMethod = 'smooth',
sqn = FALSE) # Run cqn with specified depths and with no quantile normalisation
datExpr = cqn.dat$y + cqn.dat$offset # Get the log2(Normalised RPKM) values
# Filter out genes with low counts (filter 43406)
pres = apply(datExpr>1, 1, sum)
to_keep = pres > 0.5 * ncol(datExpr)
datExpr = datExpr[to_keep,]
datExpr_post_Norm = datExpr %>% data.frame
Regions: Frontal, Temporal, Parietal and Occipital
Similar behaviour in all regions
datExpr = datExpr_post_Norm # Should be equal, jut in case
p = list()
for(lobe in names(table(datMeta$Brain_lobe))){
datExpr_lobe = datExpr %>% dplyr::select(which(datMeta$Brain_lobe==lobe))
ASD_vs_CTL = make_ASD_vs_CTL_df(datExpr_lobe, lobe)
plot = ggplotly(ggplot(ASD_vs_CTL, aes(`gene-score`, abs(mean_diff), fill=`gene-score`)) +
geom_boxplot() + theme_minimal() +
scale_fill_manual(values=gg_colour_hue(7)) +
theme(legend.position = 'none'))
p[lobe] = list(plot)
}
subplot(p[[1]], p[[2]], p[[3]], p[[4]], nrows=2)